ANTI-CHOLESTEROL ACTIVITY TEST OF N-HEXANE, METHANOL AND ETHANOL EXTRACTS ETHANOL EXTRACT OF LEMON PEEL WITH UV-VIS SPECTROPHOOMETRY METHOD
Abstract
Cholestrol is a fatty substance that is in and produced by the liver and is needed by the body.However, if cholesterol in the blood is too high it can cause blockages in blood vessels that can trigger various other types of more serious diseases such as heart disease, stroke and high blood pressure (inflammation of the pancreas organ), diabetes mellitus, thyrod disorders, etc. Lemon peel can reduce cholesterol levels in the body. Because lemon peel contains flavonoid polyphenols, which are very active in lowering cholesterol. The purpose of this study was to determine the anti-cholesterol activity and determine the EC50 value of N-Hexane partition, methanol and ethanol extracts of lemon peel (Citrus limon) invitro. This type of research is descriptive quantitative analysis method using UV-Vis spectrophotometry at a wavelength of 665.0 nm with a concentration series of test samples 150 ppm, 300 ppm, 450 ppm, 600 ppm, 750 ppm. The research data showed that the level of cholesterol reduction was directly proportional to the increase in concentration in the sample. The EC50 value of n-hexane partition was 7.6 ppm, methanol partition was 108.53 ppm and ethanol extract was 194.9231 ppm. Conclusion among the three partitions, n-hexane extract is the most effective to reduce cholesterol.
References
Alfian HK, 2017, POTENSI
ANTIOKSIDAN EKSTRAK KULIT
DAN PERASAN DAGING BUAH
LEMON (Citrus Lemon) LOKAL DAN
IMPOR, Jurnal UMJ.
Andini, et al, 2019, Uji Aktifitas antioksidan
dan toksisitas ekstraks methanol
beberapa jenis daun benalu, Volume
Hartanto,et al, 2019, "Potensi ekstrak etanol
kulit jeruk lemon (Citrus limon L.)
sebagai obat alternatif
hiperkolesterolemia pada tikus wistar
hiperglikemik." Kartika: Jurnal
Ilmiah Farmasi.
Harborne, 1996, Analisis Flavonoid,
alkoloid, kuinon dan terpenoid pada
tanaman herbarium. laut, Jurnal
Biologi Covid 19.
Gritter, 1991, Metode Ekstraksi, Jurnal
Biologi Covid 19.
Lutfiyati, Iesyi, et al, 2019 "Uji Aktivitas
Antikolesterol Partisi N-Heksana,
Metanol Dan Ekstrak Etanol Kulit
Jeruk Nipis (Citrus Aurantiifolia)
VOLUME 10 NO 01 JUNI 2023 JURNAL MEDIKA CENDIKIA
Secara In Vitro." Prosiding Seminar
Nasional Kesehatan. Vol. 1. 2021.
Richmond, 2004, Pengaruh pencahayaan
terhadap Kandungan Pigmen
Tetraselmis chuii sebagai Sumber
Antioksidan Alami. Nada Kristiani
Ginting, Sri Sedjati*, Endang
Supriyantini, Ali Ridl. Departemen
Ilmu Kelautan dan Perikanan.
Maulida, et al, 2018, "Pengaruh Rasio
Kolesterol Total terhadap High
Density Lipop rotein (HDL) pada
Kejad ian stroke Iskemik.
Soleha, Maratu, 2012, "Kadar kolesterol
tinggi dan faktor-faktor yang
berpengaruh terhadap kadar
kolesterol darah." Jurnal Biotek
Medisiana Indonesia 1.2 (2012): 85-
Rika P, et al, 2019), KARAKTERISASI
SIMPLISIA DAN SKRINING
FITOKIMIA SERTA ANALISIS
SECARA KLT (Kromatografi Lapis
Tipis) DAUN DAN KULIT BUAH
JERUK LEMON (Citrus limon (L.)
Burm.f.), Farmasi Stikes Imelda.
Sigarlaki,et al, 2016),"Pengaruh pemberian
buah naga merah (Hylocereus
polyrhizus) terhadap kadar kolesterol
total." Jurnal Majority 5.5.
Copyright (c) 2023 Titin Supriatin, N. Ai Erlinawati, Mila Mila

This work is licensed under a Creative Commons Attribution 4.0 International License.